| 產(chǎn)品編號 | bs-2594R |
| 英文名稱 | IL-1R1 Rabbit pAb |
| 中文名稱 | 白介素1受體1抗體 |
| 別 名 | Antigen CD121a; CD121 antigen-like family member A; CD121A; CD121a antigen; D2S1473; IL 1; IL 1R 1; IL 1R alpha; IL 1RT1; IL-1R-1; IL-1R-alpha; IL-1RT-1; IL-1RT1; IL1 inhibitor; IL1R; IL1R1; IL1R1_HUMAN; IL1RA; IL1RN; IL1RT1; Interleukin 1 receptor alpha |
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Specific References (3) | bs-2594R has been referenced in 3 publications.
[IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078 WB ; Mouse.
[IF=5.682] Pan, Jingrui. et al. Transmission of NLRP3-IL-1β Signals in Cerebral Ischemia and Reperfusion Injury: from Microglia to Adjacent Neuron and Endothelial Cells via IL-1β/IL-1R1/TRAF6. MOL NEUROBIOL. 2023 Jan;:1-18 WB,IF ; Rat.
[IF=4.427] Zhang?H et al. Aluminum trichloride-induced hippocampal inflammatory lesions are associated with IL-1β-activated IL-1 signaling pathway in developing rats. Chemosphere. 2018 Jul;203:170-178. WB ; Rat.
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| 研究領(lǐng)域 | 心血管 免疫學(xué) 干細(xì)胞 淋巴細(xì)胞 t-淋巴細(xì)胞 細(xì)胞因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-1R1: 481-569/569 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq]. Function: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Subunit: The interleukin-1 receptor complex is a heterodimer of IL1R1 and IL1RAP. Subcellular Location: Membrane; Single-pass type I membrane protein. Cell membrane (Probable). Secreted. Post-translational modifications: A soluble form (sIL1R1) is probably produced by proteolytic cleavage at the cell surface (shedding). Similarity: Belongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain. SWISS: P14778 Gene ID: 3554 Database links: Entrez Gene: 3554 Human Entrez Gene: 16177 Mouse Omim: 147810 Human SwissProt: P14778 Human SwissProt: P13504 Mouse Unigene: 516249 Human Unigene: 701982 Human Unigene: 896 Mouse Unigene: 9758 Rat IL-1RⅠ屬于新的信號受體家族Toll信號轉(zhuǎn)導(dǎo)通路蛋白，IL-1RⅠ在免疫和炎癥反應(yīng)的研究中占重要地位，是在很多感染 、炎癥性致病機(jī)制中起決定性作用的細(xì)胞因子，幾乎可作用于全身各種細(xì)胞，IL-1 與IL-1R1的結(jié)合可導(dǎo)致大量與免疫反應(yīng)和炎癥相關(guān)基因的表達(dá) ，包括細(xì)胞因子、細(xì)胞因子受體、急性期反應(yīng)物、生長因子、胞外基質(zhì)、粘附分子等。IL-1R1/NF-κB信號通路是機(jī)體免疫反應(yīng)中十分重要的組成部分。白介素1受體I型 Interleukin 1 receptor I(IL-1RI) |
| 產(chǎn)品圖片 |
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-IL-1R1 (bs-2594R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-IL-1R1 antibody (bs-2594R);
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol overnight at 4℃ . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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