| 產(chǎn)品編號(hào) | bs-2389R |
| 英文名稱(chēng) | Coxsackie Adenovirus Receptor Rabbit pAb |
| 中文名稱(chēng) | 柯薩奇病毒蛋白受體B抗體 |
| 別 名 | 46 kD coxsackievirus and adenovirus receptor CAR protein; 46 kD coxsackievirus and adenovirus receptor protein; CAR; CAR4/6; Coxsackie virus and adenovirus receptor; Coxsackie virus B receptor; Coxsackievirus and adenovirus receptor; Coxsackievirus B aden |
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Specific References (4) | bs-2389R has been referenced in 4 publications.
[IF=14.188] Jae Won Song. et al. Isolation and Genomic Analysis of Single Circulating Tumor Cell Using Human Telomerase Reverse Transcriptase and Desmoglein-2. 2022 Jan 17 WB ; Human.
[IF=4.717] Morio Yamazaki. et al. Soluble JAM-C Ectodomain Serves as the Niche for Adipose-Derived Stromal/Stem Cells. Biomedicines. 2021 Mar;9(3):278 WB,IHC ; Mouse.
[IF=3.51] Wang MJ et al. Baicalin Inhibits Coxsackievirus B3 Replication by Reducing Cellular Lipid Synthesis. Am J Chin Med. 2020;48(1):143-160. WB ; Human.
[IF=2.598] Mokhashi N et al. Transduction Efficiency of Adenovirus Vectors in Endothelial Cells and Vascular Smooth Muscle Cells. J Cardiovasc Pharmacol. 2020 Jun;75(6):603-607. IHF&WB ; Rat.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 微生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞骨架 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Coxsackie Adenovirus Receptor: 21-120/365 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Coxsackie and adenovirus receptor (CAR), also known as CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity. It is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.It is proposed to function as a homophilic cell adhesion molecule and recruits MPDZ to intercellular contact sites. CAR is probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN. Function: Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN. Subunit: Monomer. Probably homodimer formed by 2 molecules on adjacent cells. Interacts with LNX, MPDZ, BAIAP1, DLG4, PRKCABP, TJP1 and CTNNB1. Secreted isoform 3, isoform 4 and isoform 5 can interact with the extracellular domain of the receptor. Interacts with adenovirus subgroup A, C, D, E and F fiber proteins as well as coxsackievirus B1, B2, B3, B4, B5 and B6 capsid proteins and acts as a receptor for these viruses. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell junction, adherens junction. Basolateral cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. Isoform 2: Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell junction, adherens junction. Basolateral cell membrane; Single-pass membrane protein. Note=In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. Isoform 3: Secreted. Isoform 4: Secreted. Isoform 5: Secreted. Tissue Specificity: Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. Post-translational modifications: N-glycosylated (By similarity). Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P78310 Gene ID: 1525 Database links: Entrez Gene: 1525 Human Entrez Gene: 13052 Mouse Entrez Gene: 100174624 Orangutan Entrez Gene: 100302411 Rabbit Omim: 602621 Human SwissProt: P78310 Human SwissProt: P97792 Mouse SwissProt: Q5R764 Orangutan Unigene: 634837 Human Unigene: 66222 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Coxsackie Adenovirus Receptor) Polyclonal Antibody, Unconjugated (bs-2389R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(Blue):A549 (fixed with 2% paraformaldehyde for 10 min at 37℃). Primary Antibody:Rabbit Anti-Coxsackie Adenovirus Receptor antibody (bs-2389R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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