| 產(chǎn)品編號(hào) | bs-5154R |
| 英文名稱 | LSP1 Rabbit pAb |
| 中文名稱 | 白細(xì)胞F肌動(dòng)蛋白結(jié)合蛋白抗體 |
| 別 名 | 47 kDa actin binding protein; 47 kDa actin-binding protein; 52 kDa phosphoprotein; F actin binding and cytoskeleton associated protein; Leufactin(leukocyte F-actin binding protein); Leufactin; Leukocyte F actin binding protein; Leukocyte specific protein OTTHUMP00000014138; OTTHUMP00000014139; OTTHUMP00000014140; OTTHUMP00000069612; OTTHUMP00000069614; OTTHUMP00000069615; OTTHUMP00000069616; |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 結(jié)合蛋白 淋巴細(xì)胞 t-淋巴細(xì)胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LSP1: 241-339/339 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Function: May play a role in mediating neutrophil activation and chemotaxis (By similarity). Subunit: Binds actin. Subcellular Location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Tissue Specificity: Activated T-lymphocytes. Post-translational modifications: Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis (By similarity). SWISS: P33241 Gene ID: 4046 Database links: Entrez Gene: 4046 Human Entrez Gene: 16985 Mouse Omim: 153432 Human SwissProt: P33241 Human SwissProt: P19973 Mouse Unigene: 56729 Human Unigene: 234003 Mouse Unigene: 101608 Rat |
| 產(chǎn)品圖片 |
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- LSP1 (bs-5154R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 54 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LSP1 Polyclonal Antibody, Unconjugated(bs-5154R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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