| 產(chǎn)品編號 | bs-10296R |
| 英文名稱 | Claudin 5 Rabbit pAb |
| 中文名稱 | 緊密連接蛋白5抗體 |
| 別 名 | AWAL; BEC 1; BEC1; Claudin 5(transmembrane protein deleted in velocardiofacial syndrome); Claudin5; Claudin-5; CLDN 5; CLDN5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofac |
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Specific References (8) | bs-10296R has been referenced in 8 publications.
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| 研究領(lǐng)域 | 信號轉(zhuǎn)導(dǎo) 細胞粘附分子 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 細胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Claudin 5: 29-81/218 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008] Function: Plays a major role in tight junction-specific obliteration of the intercellular space. Subunit: Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ. Subcellular Location: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. Similarity: Belongs to the claudin family. SWISS: O00501 Gene ID: 7122 Database links: Entrez Gene: 7122 Human Entrez Gene: 12741 Mouse Omim: 602101 Human SwissProt: O00501 Human SwissProt: O54942 Mouse Unigene: 505337 Human Unigene: 22768 Mouse
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| 產(chǎn)品圖片 |
Protein: lung(mouse) lysates at 45ug;
Primary: Anti-Claudin 5 (bs-10296R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size :23 kD
Observed band size :23kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 5) Polyclonal Antibody, Unconjugated (bs-10296R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-Claudin 5 antibody (bs-10296R)
Dilution:2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
