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Histone H3.1 Rabbit pAb (bs-17422R)

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產(chǎn)品編號	bs-17422R
英文名稱	Histone H3.1 Rabbit pAb
中文名稱	組蛋白H3.1抗體
別名	H3 histone family, member A; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member E; H3 histone family, member F; H3 histone family, member G; H3 histone family, member H; H3 histone family, memb
	Specific References (12) \| bs-17422R has been referenced in 12 publications. [IF=16.988] Wu Yutong. et al. Osteoclast-Derived Apoptotic Bodies Inhibit Naive Cd8 T Cell Activation via Siglec15 Promoting Breast Cancer Secondary Metastasis. Cell Reports Medicine. 2022 Nov 03 WB ; Mouse. PubMed:10.2139/ssrn.4255228 [IF=14.976] Qinyu Ma. et al. Small extracellular vesicles deliver osteolytic effectors and mediate cancer‐induced osteolysis in bone metastatic niche. J Extracell Vesicles. 2021 Feb;10(4):e12068 WB ; Mouse. PubMed:33659051 [IF=7.109] Wu Yutong. et al. Reduced osteoclast-derived apoptotic bodies in bone marrow characterizes the pathological progression of osteoporosis. CELL DEATH DISCOV. 2023 Apr;9(1):1-9 WB ; Mouse. PubMed:37185334 [IF=7.032] Mengmeng Liang. et al. Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1. Mol Ther-Nucl Acids. 2021 Mar;23:1191 WB ; Mouse. PubMed:33664997 [IF=6.953] Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-BHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453 WB ; Human. PubMed:35339497 [IF=4.85] Yutong Wu. et al. Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. CELL SIGNAL. 2022 Dec;:110549 WB ; Mouse. PubMed:36464103 [IF=3.188] Li Y et al. Silencing of LncRNA Steroid Receptor RNA Activator attenuates polycystic ovary syndrome in Mice. (2018)Biochimie; Nov 1;157:48-56. WB ; Mouse. PubMed:30391287 [IF=2.561] Liu B et al. Biglycan promotes the chemotherapy resistance of colon cancer by activating NF-κB signal transduction.Mol Cell Biochem. 2018 May 14. WB ; Human. PubMed:29761248 [IF=2.332] Liu J et al. Molecular detection of Hsp90 inhibitor suppressing PCV2 replication in host cells. Microb Pathog. 2019 Apr 25;132:51-58. WB ; Pig. PubMed:31028862 [IF=1.98] Fang D et al. Overexpression of Biglycan is Associated with Resistance to Rapamycin in Human WERI-Rb-1 Retinoblastoma Cells by Inducing the Activation of the Phosphatidylinositol 3-Kinases (PI3K)/Akt/Nuclear Factor kappa B (NF-κB) Signaling Pathway. Med Sci Monit. 2019 Sep 4;25:6639-6648. WB ; Human. PubMed:31483776 [IF=1.918] Dong Y et al. Quercetin Inhibits the Proliferation and Metastasis of Human Non-Small Cell Lung Cancer Cell Line: The Key Role of Src-Mediated Fibroblast Growth Factor-Inducible 14 (Fn14)/ Nuclear Factor kappa B (NF-κB) pathway. Med Sci Monit . 2020 Mar 30;26:e920537. WB ; human. PubMed:32225128 [IF=1.894] Wang et al. Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-κB Signaling. (2018) Med.Sci.Monit. 24:1282-1294 WB ; Human. PubMed:29500337
研究領域	細胞生物表觀遺傳學
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse,Chimpanzee)
產(chǎn)品應用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	15 kDa
檢測分子量
細胞定位	細胞核
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Histone H3.1: 71-136/136
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] Function: Histone H3.1 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodelling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3 H4 heterotetramer and two H2A H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular Location: Nuclear Similarity: Belongs to the histone H3 family. SWISS: Q71DI3 Gene ID: 8968 Database links: Entrez Gene: 8350 Human Entrez Gene: 8351 Human Entrez Gene: 8352 Human Entrez Gene: 8353 Human Entrez Gene: 8354 Human Entrez Gene: 8355 Human Entrez Gene: 8356 Human Entrez Gene: 8357 Human Entrez Gene: 8358 Human Entrez Gene: 8968 Human Entrez Gene: 319148 Mouse Entrez Gene: 319151 Mouse Entrez Gene: 319152 Mouse Entrez Gene: 319153 Mouse Entrez Gene: 360198 Mouse Entrez Gene: 97908 Mouse Omim: 602810 Human Omim: 602811 Human Omim: 602812 Human Omim: 602813 Human Omim: 602814 Human Omim: 602815 Human Omim: 602816 Human Omim: 602817 Human Omim: 602818 Human Omim: 602819 Human SwissProt: P68431 Human SwissProt: P68433 Mouse Unigene: 132854 Human Unigene: 247813 Human Unigene: 247814 Human Unigene: 248176 Human Unigene: 443021 Human Unigene: 484990 Human Unigene: 532144 Human Unigene: 533292 Human Unigene: 546315 Human Unigene: 586261 Human Unigene: 591778 Human Unigene: 626666 Human Unigene: 221301 Mouse Unigene: 261657 Mouse Unigene: 377874 Mouse Unigene: 390558 Mouse
產(chǎn)品圖片	Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Thymus tissue lysates Lane 3: Rat Thymus tissue lysates Lane 4: Human HeLa cell lysates Lane 5: Human SH-SY5Y cell lysates Lane 6: Human 293T cell lysates Lane 7: Human U251 cell lysates Primary: Anti-Histone H3.1 (bs-17422R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 17 kDa Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3.1) Polyclonal Antibody, Unconjugated (bs-17422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關于肽鏈的設計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序

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