產(chǎn)品編號	bsm-33190M
英文名稱	Caspase 8 Mouse mAb
中文名稱	半胱氨酸蛋白酶8單克隆抗體
別名	Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CA
	Specific References (9) \| bsm-33190M has been referenced in 9 publications. [IF=6.291] Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-κB pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166 WB ; Bovine. PubMed:35030520 [IF=5.89] Rentao Zhang. et al. Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and Lactiplantibacillus plantarum YT013. FRONT BIOENG BIOTECH. 2022; 10: 898240 WB ; Human. PubMed:35677304 [IF=5.162] Serag Eldin I. Elbehairi. et al. Chitosan nano-vehicles as biocompatible delivering tools for a new Ag(I)curcuminoid-Gboxin analog complex in cancer and inflammation therapy. Int J Biol Macromol. 2020 Dec;165:2750 WB ; Human. PubMed:10.1016/j.ijbiomac.2020.10.153 [IF=5.123] Rentao Zhang. et al. Exopolysaccharide from Lactiplantibacillus plantarum YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells. Fermentation-Basel. 2023 Jun;9(6):539 WB ; Human. PubMed:10.3390/fermentation9060539 [IF=5.016] Min-Lin He. et al. Screening and characterization of a novel efficient tumor cell-targeting peptide derived from insulin-like growth factor binding proteins. J DRUG TARGET. 2023 Mar 28 WB ; Human. PubMed:36974745 [IF=4.462] Zhao W et al. Junduqing extractive promotes the apoptosis of nasopharyngeal carcinoma cells through down-regulating Mcl-1 and Bcl-xL and up-regulating Caspase-3, Caspase-8 and Caspase-9. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3904-3912. WB ; Human. PubMed:31566021 [IF=3.448] Xu B et al. Excessive?mechanical?stress?induces?chondrocyte?apoptosis?through?TRPV4?in an?anteriorcruciate?ligament-transected?rat?osteoarthritis?model. Life Sci. 2019 Jul 1;228:158-166. WB ; Rat. PubMed:31055086 [IF=3.417] Lei Xiang. et al. Antitumor effects of curcumin on the proliferation, migration and apoptosis of human colorectal carcinoma HCT?116 cells. Oncol Rep. 2020 Nov;44(5):1997-2008 WB ; Human. PubMed:33000266 [IF=3.067] Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. WB ; Mouse. PubMed:31491480
研究領(lǐng)域	細(xì)胞生物神經(jīng)生物學(xué) 細(xì)胞凋亡
抗體來源	Mouse
克隆類型	Monoclonal
克隆號	4G12
交叉反應(yīng)	Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用	WB=1:2000-5000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	43/57 kDa
檢測分子量	43
細(xì)胞定位	細(xì)胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	Recombinant human Caspase 8 Protein
亞型	IgG
純化方法	affinity purified by Protein G
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Function: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-\|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Subcellular Location: Cytoplasm. Tissue Specificity: Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. DISEASE: Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. Similarity: Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains. SWISS: Q14790 Gene ID: 841 Database links: Entrez Gene: 841 Human Entrez Gene: 12370 Mouse Entrez Gene: 54474 Rat Entrez Gene: 64044 Rat Omim: 601763 Human SwissProt: Q14790 Human SwissProt: O89110 Mouse SwissProt: Q9JHX4 Rat Unigene: 599762 Human Unigene: 655983 Human Unigene: 336851 Mouse 半胱氨酸蛋白酶-8，在凋亡執(zhí)行階段起中心作用。
產(chǎn)品圖片	Sample: Hela(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41'43'55 kD Observed band size: 43 kD Sample: 293T-UV(Human) Cell Lysate at 30 ug U251-UV(Human) Cell Lysate at 30 ug Primary: Anti-Caspase 8 (bsm-33190M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41’43’55 kD Observed band size: 43 kD Sample: Stomach(Mouse) Lysate at 40 ug Spleen(Mouse) Lysate at 40 ug Lymph node(Mouse) Lysate at 40 ug Urinary bladder(Mouse) Lysate at 40 ug Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41'43'55 kD Observed band size: 55'53D

	AbBy Fluor 350 標(biāo)記
	AbBy Fluor 488 標(biāo)記
	AbBy Fluor 555 標(biāo)記
	AbBy Fluor 594 標(biāo)記
	AbBy Fluor 647 標(biāo)記
	AP 標(biāo)記
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	HRP 標(biāo)記
	PE 標(biāo)記
	PE-Cy3 標(biāo)記
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	免疫組化常用試劑
	免疫印跡常用試劑
	各種標(biāo)記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計
	9、多肽的溶解與保存
	10、酶標(biāo)抗體效價測定程序