| 產(chǎn)品編號 | bs-23272R |
| 英文名稱 | Phospho-MKP1 (Ser296) Rabbit pAb |
| 中文名稱 | 磷酸化絲裂原活化蛋白激酶磷酸酶-1抗體 |
| 別 名 | DUSP1(phospho S296); MKP1(phospho Ser296); MKP1(phospho S296); Mitogen activated protein kinase phosphatase 1; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP 1; EC 3.1 |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised monomethylpeptide derived from human MKP1 around the phosphorylation site of Ser296: II(p-S)PN |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Function: Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185'. Tissue Specificity: Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain. SWISS: P28562 Gene ID: 1843 Database links: Entrez Gene: 1843 Human Entrez Gene: 19252 Mouse Omim: 600714 Human SwissProt: P28562 Human SwissProt: P28563 Mouse Unigene: 171695 Human Unigene: 239041 Mouse Unigene: 98260 Rat MKPs是一類絲氨酸/蘇氨酸和酪氨酸雙重底物特異性的磷酸酶 ,對于絲裂素活化蛋白激酶活性的調(diào)節(jié)起著十分重要的作用，可使絲裂素活化蛋白激酶上的蘇氨酸/酪氨酸去磷酸化失活。目前研究發(fā)現(xiàn)MKPs分別有MKP-1、MKP-2、MKP-3及MKP4-6。 MKPs受MAPK信號通路中多種成分的誘導(dǎo) ,決定了它與MAPK之間作用的特異性。 通過去磷酸化作用調(diào)節(jié)MAPK信號途徑的活性 ,確保了細(xì)胞內(nèi)信號的精確傳遞 ,參與了多種主要的細(xì)胞功能的調(diào)節(jié)。 |
