| 產(chǎn)品編號 | BH0010 |
| 英文名稱 | GFAP(ready to use) Mouse mAb |
| 中文名稱 | GFAP單克隆抗體（工作液） |
| 別 名 | Astrocyte; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN. 膠質(zhì)纖維酸性蛋白單克隆抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 神經(jīng)生物學(xué) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 7D8 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 免 疫 原 | Recombinant mouse GFAP full length, |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 1% BSA and 0.02% Proclin300. |
| 保存條件 | Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells during development. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker. Function: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Subunit: Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus). Subcellular Location: Cytoplasm. Note=Associated with intermediate filaments. Tissue Specificity: Expressed in cells lacking fibronectin. Post-translational modifications: Phosphorylated by PKN1. DISEASE: Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Similarity: Belongs to the intermediate filament family. SWISS: P03995 Gene ID: 14580 Database links: Entrez Gene: 2670 Human Omim: 137780 Human SwissProt: P14136 Human 星形膠質(zhì)細胞標志物 （Astrocyte Marker） GFAP是一個56kDa的中間絲蛋白（intermediate filament，IF），在中樞神經(jīng)系統(tǒng)發(fā)育期是一個特異性的標志物，以區(qū)別星形細胞和其它膠質(zhì)細胞。GFAP表達在皮層和海馬,急、慢性皮質(zhì)酮治療時表達減少。 GFAP可以和人、大鼠、小鼠的GFAP反應(yīng)，在正常和腫瘤性的星形膠質(zhì)細胞陽性表達，而神經(jīng)節(jié)細胞、神經(jīng)元、成纖維細胞、少突膠質(zhì)細胞和這些細胞來源的腫瘤細胞陰性表達，主要用于星形膠質(zhì)瘤等中樞神經(jīng)系統(tǒng)腫瘤的診斷和鑒別診斷,GFAP的缺乏可導(dǎo)致AD病。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP(ready to use) Monoclonal Antibody, Unconjugated(BH0010) at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP(ready to use) Monoclonal Antibody, Unconjugated(BH0010) at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP(ready to use) Monoclonal Antibody, Unconjugated(BH0010) at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
