| 產(chǎn)品編號 | bs-41345R |
| 英文名稱 | SPARC Rabbit pAb |
| 中文名稱 | 富含半胱氨酸的酸性分泌蛋白抗體 |
| 別 名 | AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich(osteonectin) |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 干細胞 細胞周期蛋白 細胞骨架 細胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human SPARC protein: 18-303/303 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]. Function: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane. Similarity: Belongs to the SPARC family. Contains 1 EF-hand domain. Contains 1 follistatin-like domain. Contains 1 Kazal-like domain. SWISS: P09486 Gene ID: 6678 Database links: Entrez Gene: 6678 Human Omim: 182120 Human SwissProt: P09486 Human Unigene: 111779 Human Unigene: 708558 Human SPARC是從多方面調(diào)節(jié)細胞功能的細胞外基質(zhì)蛋白，SPARC蛋白在人組織中廣泛分布與組織重建和腫瘤有關(guān)。SPARC在多種腫瘤中呈高表達. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Mouse B16 cell lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-SPARC (bs-41345R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC ) Polyclonal Antibody, Unconjugated (bs-41345R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC ) Polyclonal Antibody, Unconjugated (bs-41345R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag (Cat. bs-41345P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human SPARC Antibody, the EC50 is 72.97 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag (Cat. bs-41345P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human SPARC Antibody, the EC50 is 21.47 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
